Regulatory

Part:BBa_K4806013:Design

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-09-12)


AβSAP(i)-promotor for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 530
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Chlamydomonas reinhardtii and compatible with Phytobricks/MoClo parts.

Source

Einhaus A, Baier T, Rosenstengel M, Freudenberg R, Kruse O. Rational Promoter Engineering Enables Robust Terpene Production in Microalgae. ACS Synthetic Biology. 2021;10(4):847–856.

References